Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Enhancing Effect of Bile Salts on Gastrointestinal Absorption of Insulin

Amir Jalali^1,2, Eskandar Moghimipour^3,4 , Abbas Akhgari⁴

¹Department of Pharmacology and Toxicology; ²Toxicology Research Center; ³Molecular and Cellular Research Center; ⁴Nanotechnology Research Center, Faculty of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

For correspondence:- Eskandar Moghimipour Email: moghimipour@yahoo.com Tel:+986113738378

Received: 21 October 2013 Accepted: 11 September 2014 Published: 24 November 2014

Citation: Jalali A, Moghimipour E, Akhgari A. Enhancing Effect of Bile Salts on Gastrointestinal Absorption of Insulin. Trop J Pharm Res 2014; 13(11):1797-1802 doi: 10.4314/tjpr.v13i11.4

© 2014 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the effect of co-administration of two absorption enhancing bile salts, sodium glycocholate (NaGc) and sodium salicylate (NaSal), on insulin absorption via intestinal targeted delivery system.

Methods: Insulin (10 IU/kg), associated with and without absorption enhancers (5 % enhancer solution of NaGc or NaSal), was administered to the duodenum, jejunum, and ileum part of the diabetic rat's gastrointestinal (GI) tract by surgical technique. The insulin absorbed from the GI tract was evaluated by its hypoglycemic effect at 45 and 60 min post-administration.

Results: The results showed that insulin formulations containing NaGc or NaSal administered into the duodenum and with little quantity in the jejunum decreased blood glucose levels, compared to the reference formulations (p < 0.05). It was also observed that formulations containing cellulose acetate phthalate (CAP) protectors and enhancers may protect insulin during transit through the stomach for 180 min.

Conclusion: Thus, the results of this study demonstrate that duodenum-specific delivery of insulin with NaGc and NaSal was achievable by oral administration compared to the other parts of small intestine. Furthermore, NaGc has a greater enhancing effect on duodenal, and to some extent, jejunal absorption of insulin.

Keywords: Bile salts, Sodium glycocholate, Sodium salicylate, Insulin, Gastrointestinal administration